Molecular Genotyping

Genotyping: Quality control and verification of contaminants.

The genotyping methodology for microorganisms starts with the extraction of DNA directly from isolated colonies followed by the amplification of nucleic acids (PCR) from conserved regions of DNA between microorganisms.

The PCR product (amplicon) is sequenced and the result compared in genomic banks giving a fast and reliable result.

Process

Chapter 1113 of the USP (United States Pharmacopeia) recommends that research down to the species level be carried out by molecular genotyping .

Origin Identification

O rápido ciclo de vida dos microrganismos aliado à propensão a erros de suas enzimas internas acaba gerando mudanças que podem comprometer o sistema produtivo. Para certificar que os microrganismos utilizados no processo industrial e na composição de produtos sejam iguais aos especificados no rótulo e no registro inicial do produto, a Gentros oferece o serviço de identificação de origem a partir do processo de Genotipagem Molecular.

A análise é realizada pela técnica de PCR- RFLP permitindo assegurar a qualidade do produto e do processo produtivo.

The National Health Surveillance Agency (ANVISA) regulated the labeling of allergenic substances in foods, complementing Resolution RDC nº 259/2002, which approves the technical regulation for the labeling of packaged foods, and Law nº 10.674 / 2003, which established the obligation on the presence of gluten in food products.

Allergen

For most allergenic foods, there is no information on the safe dose limit for their intake, so the only option for allergic people is to avoid food and its derivatives entirely, making food labeling an essential ally for the population. Gentros offers safe and fast PCR-based tests for detection and quantification of allergenic substances in fresh or processed foods.